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Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen  Vibrio cholerae , which secretes various proteinaceous effectors at different stages of its lifecycle. Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology. PglL Vc , a predicted oligosaccharyltransferase of  V. cholerae , has been recently shown to exhibit  O -glycosylation activity with relaxed glycan specificity in an engineered  Escherichia coli  system. By engineering  V. cholerae  strains to express a defined, undecaprenyl diphosphate-linked glycoform precursor, we confirmed functional  O -linked protein glycosylation activity of PglL Vc  in  V. cholerae . We demonstrate that PglL Vc  is required for the glycosylation of multiple  V. cholerae  proteins, including periplasmic chaperones such as DegP, that are required for efficient type II-dependent secretion. Moreover, defined deletion mutants and complementation strains provided first insights into the physiological role of  O -linked protein glycosylation in  V. cholerae . RbmD, a protein with structural similarities to PglL Vc  and other established oligosaccharyltransferases (OTases), was also included in this phenotypical characterization. Remarkably, presence or absence of PglL Vc  and RbmD impacts the secretion of proteins via the type II secretion system (T2SS). This is highlighted by altered cholera toxin (CT) secretion, chitin utilization and biofilm formation observed in Δ pglL  Vc  and Δ rbmD  single or double mutants. This work thus establishes a unique connection between broad spectrum  O -linked protein glycosylation and the efficacy of type II-dependent protein secretion critical to the pathogen’s lifecycle.

A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes in Vibrio cholerae