English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Listeria monocytogenes  is a Gram-positive bacterium that can be found in a broad range of environments, including soil, food, animals, and humans.  L. monocytogenes  can cause a foodborne disease manifesting as sepsis and meningo-encephalitis. To evaluate signals of selection within the core genome of neuroinvasive  L. monocytogenes  strains, we sequenced 122  L. monocytogenes  strains from cerebrospinal fluid (CSF) of Dutch meningitis patients and performed a genome-wide analysis using Tajima’s D and ω (dN/dS). We also evaluated the residual variation intolerance score (RVIS), a computationally less demanding methodology, to identify loci under selection. Results show that the large genetic distance between the listerial lineages influences the Tajima’s D and ω (dN/dS) outcome. Within genetic lineages we detected signals of selection in 6 of 2327 loci (<1%), which were replicated in an external cohort of 105 listerial CSF isolates from France. Functions of identified loci under selection were within metabolism pathways ( lmo2476 , encoding aldose 1-epimerase), putative antimicrobial resistance mechanisms ( lmo1855 , encoding PBPD3), and virulence factors ( lmo0549 , internalin-like protein;  lmo1482 , encoding comEC). RVIS over the two genetic lineages showed signals of selection in internalin-like proteins loci potentially involved in pathogen-host interaction ( lmo0549 ,  lmo0610 , and  lmo1290 ). Our results show that RVIS can be used to detect bacterial loci under selection.

Residual Variation Intolerance Score Detects Loci Under Selection in Neuroinvasive Listeria monocytogenes