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Many  Burkholderia  spp. produce  in vitro  secondary metabolites with relevant biological activities and potential practical applications.  Burkholderia gladioli  pv.  agaricicola  ( Bga ) possess promising biological activities regulated by  N -Acyl homoserine lactones ( N .AHLs) based quorum sensing (QS) mechanism. In the current study,  N .AHLs-deficient ( ICMP11096glad- I) and  N .AHLs-complemented ( ICMP11096glad- IR) mutants were constructed in which the gene coding for AHL synthase was inactivated by allelic exchange in  glad  I mutant strain. The aims of this research were to (i) assess the antagonistic activity of the wild type (WT) and the  glad- I mutant of  Bga  against  Bacillus megaterium  (G+ve) and  Escherichia coli  (G−ve), (ii) screen their hydrolytic enzymes and hemolytic substances, (iii) monitor the pathogenic effect against  Agaricus bisporus , and finally (iv) analyze the bioactive secondary metabolites produced by WT and mutant strain using high performance liquid chromatography (HPLC). Results showed that  N. AHLs-deficient mutant exhibited high reduction of antagonistic activity against the tested microorganisms and notable reduction of chitinolytic, proteolytic and glucanolytic activities and complete absence of hemolytic activity, and the  glad- IR complemented mutant was able to regain the major part of these activities. Furthermore,  N .AHLs-deficient mutant strain was unable to degrade flesh cubes pseudo-tissues of  A. bisporus . On the other hand, the virulence effect of complemented mutant was like to the parental WT strain. HPLC analysis revealed that some of the single components produced by WT strain were absent in  N. AHLs-deficient mutant and others were highly reduced. The out-findings of the current research gave a spot into the regulatory role of  N .AHLs and QS phenomenon in the biological activity of  Bga  bacterium.

frontiers in microbiology

Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp.