English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The Asian citrus psyllid ( Diaphorina citri ) is a major pest of citrus trees as it transmits  Candidatus  Liberibacter asiaticus ( C Las). The composition of a host’s microbiota can affect the evolution and ecological distribution of the host. This study monitored the compositional shifts in the citrus psyllid microbiota through all the life stages (egg, nymph 1–5 stages, and adult) by next-generation sequencing (NGS) and quantitative real-time PCR. There were clear differences in both α- and β-diversity of microbiota through the psyllid life stages. Microbiota diversity was markedly higher in the nymph 2–5 stages than in the adult, egg, and nymph 1 stages. Proteobacteria were dominant in all the life stages of  D. citri , representing >97.5% of the total bacterial community, and  Candidatus  Profftella armature was the dominant genus in all the life stages. Data from the qPCR analysis showed an exponential increase in the populations of three  D. citri  endosymbionts:  Candidatus  Profftella armature,  Candidatus  Carsonella ruddii, and  Wolbachia . The gut bacterium  Pantoea  was present in all the life stages, but it was markedly higher in the nymph 2–5 stages. The microbiota composition substantially differed among the egg–nymph 1, nymphs 2–5, and adult stages. Therefore, we successfully characterized the microbiota dynamics and thus identified a microbiota shift during the life cycle of  D. citri  by 16S rRNA gene sequencing and quantitative PCR. Moreover, 16S rRNA gene sequencing suggested that  D. citri  acquired the ability to bear  C Las in the nymph 1 stage. This study enhances our understanding of microbial establishment in the developing  D. citri  and provides a reference resource for the identification of potential biocontrol approaches against this pest.

16S rRNA Gene Sequencing Reveals a Shift in the Microbiota of Diaphorina citri During the Psyllid Life Cycle