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Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The  Neurospora crassa  gene  ada-6  encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of  ada-6  to conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δ ada-6  mutant and wild type during conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δ ada-6  mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several conidiation related genes are positively regulated by ADA-6, including genes that positively regulate conidiation ( fluffy  and  acon-3 ), and genes preferentially expressed during conidial development ( eas ,  con-6 ,  con-8 ,  con-10 ,  con-13 ,  pcp-1 , and NCU9357), as the expression of these genes were lower in the Δ ada-6  mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by  ada-6  deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of  ada-6  resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of  pre-2 ,  poi-2 , and NCU05832, three key genes participating in sexual development. In both conidiation and the sexual development process, ADA-6 regulates the transcription of  cat-3  and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of  ada-6  led to hypersensitivity to oxidants H 2 O 2  and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in conidiation, sexual development, and oxidative stress response of  N. crassa .

The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa