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Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about  Cymbopogon citratus , an economically valuable medicinal plant. In this study  Cymbopogon citratus  was elected as a subject plant over the five selected plants ( Azadirachta indica ,  Plumeria obtuse ,  Sapindus mukorossi ,  Capsicum annuum  and  Phyllanthus emblica ) on the basis of antibacterial effect against dominating pathogenic species of gram positive ( Bacillus cereus, Bacillus licheniformis ) and gram negative ( Pseudomonas aeruginosa, Escherichia coli ) bacteria. Further, bioactive agents behind antibacterial potential of  Cymbopogon citratus  was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of  Cymbopogon citratus  silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C 25 –C 150  (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C 25  (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C 25  (μg/ml) and C 50  (μg/ml) noted as the MBC for  Pseudomonas aeruginosa, Escherichia coli  and  Bacillus cereus, Bacillus licheniformis , respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C 150  (μg/ml) concentration  Bacillus cereus  (20.12 ± 0.42),  Bacillus licheniformis  (22.34 ± 0.4),  Pseudomonas aeruginosa  (35.23 ± 0.46) and  Escherichia coli  (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with  ∼ 35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in  Cymbopogon citratus  extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β –Pinene)

frontiers in microbiology

Analytical Investigation of Cymbopogon citratus and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria