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Escherichia coli  ST405 is an emerging urosepsis pathogen, noted for carriage of  bla  CTX-M ,  bla  NDM , and a repertoire of virulence genes comparable with O25b:H4-ST131. Extraintestinal and multidrug resistant  E. coli  ST405 are poorly studied in Australia. Here we determined the genome sequence of a uropathogenic, multiple drug resistant  E. coli  ST405 (strain 2009-27) from the mid-stream urine of a hospital patient in Sydney, Australia, using a combination of Illumina and SMRT sequencing. The genome of strain 2009-27 assembled into two unitigs; a chromosome comprising 5,287,472 bp and an IncB/O plasmid, pSDJ2009-27, of 89,176 bp.  In silico  and phenotypic analyses showed that strain 2009-27 is a serotype O102:H6, phylogroup D ST405 resistant to ampicillin, azithromycin, kanamycin, streptomycin, trimethoprim, and sulphafurazole. The genes encoding resistance to these antibiotics reside within a novel, mobile IS 26 -flanked transposon, identified here as Tn 6242 , in the chromosomal gene  yjdA . Tn 6242  comprises four modules that each carries resistance genes flanked by IS 26 , including a class 1 integron with  dfrA17  and  aadA5  gene cassettes, a variant of Tn 6029 , and  mphA . We exploited unique genetic signatures located within Tn 6242  to identify strains of ST405 from Danish patients that also carry the transposon in the same chromosomal location. The acquisition of Tn 6242  into  yjdA  in ST405 is significant because it (i) is vertically inheritable; (ii) represents a reservoir of resistance genes that can transpose onto resident/circulating plasmids; and (iii) is a site for the capture of further IS 26 -associated resistance gene cargo.

Multidrug Resistant Uropathogenic Escherichia coli ST405 With a Novel, Composite IS26 Transposon in a Unique Chromosomal Location