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In this study, we reconstructed for the first time a genome-scale metabolic model (GSMM) of  Ganoderma lucidum  strain CGMCC5.26, termed model  i ZBM1060, containing 1060 genes, 1202 metabolites, and 1404 reactions. Important findings based on model  i ZBM1060 and its predictions are as follows: (i) The extracellular polysaccharide (EPS) biosynthetic pathway was elucidated completely. (ii) A new fermentation strategy is proposed: addition of phenylalanine increased EPS production by 32.80% in simulations and by 38.00% in experiments. (iii) Eight genes for key enzymes were proposed for EPS overproduction. Model  i ZBM1060 provides a useful platform for regulating EPS production in terms of system metabolic engineering for  G. lucidum , as well as a guide for future metabolic pathway construction of other high value-added edible/ medicinal mushroom species.

Reconstruction and Analysis of a Genome-Scale Metabolic Model of Ganoderma lucidum for Improved Extracellular Polysaccharide Production