English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The high-osmolarity glycerol response (HOG) pathway is pivotal in environmental stress response, differentiation, and virulence of  Cryptococcus neoformans , which causes fatal meningoencephalitis. A putative membrane sensor protein, Sho1, has been postulated to regulate HOG pathway, but its regulatory mechanism remains elusive. In this study, we characterized the function of Sho1 with relation to the HOG pathway in  C. neoformans . Sho1 played minor roles in osmoresistance, thermotolerance, and maintenance of membrane integrity mainly in a HOG-independent manner. However, it was dispensable for cryostress resistance, primarily mediated through the HOG pathway. A mucinlike transmembrane (TM) protein, Msb2, which interacts with Sho1 in  Saccharomyces cerevisiae , was identified in  C. neoformans , but found not to interact with Sho1.  MSB2  codeletion with  SHO1  further decreased osmoresistance and membrane integrity, but not thermotolerance, of  sho1 Δ mutant, indicating that both factors play to some level redundant but also discrete roles in  C. neoformans . Sho1 and Msb2 played redundant roles in promoting the filamentous growth in sexual differentiation in a Cpk1-independent manner, in contrast to the inhibitory effect of the HOG pathway in the process. Both factors also played redundant roles in maintaining cell wall integrity in the absence of Mpk1. Finally, Sho1 and Msb2 play distinct but complementary roles in the pulmonary virulence of  C. neoformans . Overall, Sho1 and Msb2 play complementary but distinct roles in stress response, differentiation, and pathogenicity of  C. neoformans .

Sho1 and Msb2 Play Complementary but Distinct Roles in Stress Responses, Sexual Differentiation, and Pathogenicity of Cryptococcus neoformans