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Background:  Candidiasis is a major cause of human morbidity and mortality. Human uterine cervical stem cells conditioned medium (hUCESC-CM) is obtained from stromal stem cells of the cervical transformation zone, which are in permanent contact with a wide array of potential vaginal pathogens. In previous reports we have found that hUCESC-CM has antitumor and antibacterial potential. Since  Candida  is the most prevalent yeast in the human vagina, it seems plausible that hUCESC-CM might also show activity against it.   Methods:  In a preliminary step, to evaluate if hUCESC-CM showed any activity at all on  Candida  growth,  in vitro  activities of hUCESC-CM against fluconazole-susceptible reference strains of  Candida albicans, Candida glabrata, Candida krusei , and  Candida parapsilosis  were studied with a microdilution method on RPMI 1640, using the BioScreen C microbiological incubator. Each measurement was repeated five times. The same methodology was used subsequently on fluconazole-susceptible and fluconazole-resistant  Candida  isolates from blood and vagina of those species corresponding to the reference strains of  Candida  against which activity had been detected in the previous study. Moreover, two fluconazole-resistant clinical isolates of  Candida auris  from blood and urine were also included.   Findings:   In vitro  inhibitory activity of hUCESC-CM ranged from 57.5 to 96.6% growth-reduction against fluconazole-susceptible reference strains of  Candida albicans, Candida glabrata , and  Candida parapsilosis . hUCESC-CM also reduced the growth of all fluconazole-susceptible tested vaginal isolates by more than 50%. For fluconazole-resistant isolates, growth-reduction was higher than 67% for  Candida albicans , regardless of its origin (vagina or blood). The isolate of  Candida auris  from urine with a MIC > 128 μ/ml for fluconazole was also significantly inhibited. However, hUCESC-CM was almost inactive against any of the fluconazole-resistant blood isolates of  Candida glabrata, Candida parapsilosis , and  Candida auris  tested.   Interpretation:  This is the first report about the growth-inhibiting properties of conditioned medium from human stromal stem cells against different species of  Candida . Antifungal activity of stromal stem cells depends on their site of origin, being most effective against  Candida  species most prevalent at that particular location. If confirmed in further studies, these findings might result in a completely new therapeutic approach against superficial and invasive candidiasis.

frontiers in microbiology

Antifungal Activity of the Human Uterine Cervical Stem Cells Conditioned Medium (hUCESC-CM) Against Candida albicans and Other Medically Relevant Species of Candida