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SFH1 (for  S n f 5  h omolog) protein, comprised in the RSC ( R emodels  S tructure of  C hromatin) chromatin remodeling complex, functions as a transcription factor (TF) to specifically regulate gene transcription and chromatin remodeling. As one of the well-conserved TFs in eukaryotic organisms, little is known about the roles of SFH1 protein in the filamentous fungi. In  Sclerotinia sclerotiorum , one of the notorious plant fungal pathogens, there are nine proteins predicted to contain GATA-box domain according to GATA family TF classification, among which  Sssfh1  (SS1G_01151) encodes a protein including a GATA-box domain and a SNF5 domain. Here, we characterized the roles of  Sssfh1  in the developmental process and fungal pathogenicity by using RNA interference (RNAi)-based gene silencing in  S. sclerotiorum . RNA-silenced strains with significantly reduced  Sssfh1  RNA levels exhibited slower hyphal growth and decreased reactive oxygen species (ROS) accumulation in hyphae compared to the wild-type (WT) strain. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that SsSFH1 interacts with SsMSG5, a MAPK phosphatase in  S. sclerotiorum . Furthermore,  Sssfh1 -silenced strains exhibited enhanced tolerance to NaCl and H 2 O 2 . Results of infection assays on soybean and common bean ( Phaseolus vulgaris ) leaves indicated that  Sssfh1  is required for full virulence of  S. sclerotiorum  during infection in the susceptible host plants. Collectively, our results suggest that the TF SsSFH1 is involved in growth, ROS accumulation and virulence in  S. sclerotiorum .

frontiers in microbiology

Sssfh1, a Gene Encoding a Putative Component of the RSC Chromatin Remodeling Complex, Is Involved in Hyphal Growth, Reactive Oxygen Species Accumulation, and Pathogenicity in Sclerotinia sclerotiorum