English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Many haloarchaea produce extracellular subtilisin-like proteases (halolysins) during late log phase; however, the physiological function and regulatory mechanism of growth phase-dependent production of halolysins are unknown. Halolysin SptA, the major extracellular protease of  Natrinema  sp. J7-2, is capable of intracellular self-activation to affect haloarchaeal growth. Here, we report that deletion of  sptA  leads to loss of extracellular and intracellular protease activities against azocasein and/or suc-AAPF-pNA, as well as a change in growth-phase transition of the haloarchaeon. Our results suggest that SptA is important for strain J7-2 to enter the stationary and death phases. Deletion and mutational analyses of the 5′-flanking region of  sptA  revealed two partially overlapping, semi-palindromic sequences upstream of the TATA box act as positive and negative  cis -regulatory elements, respectively, to mediate  sptA  expression in late log phase. Additionally, a negative  cis -regulatory element covering WW motif and a distant enhancer contribute to the modulation of  sptA  expression. Our results demonstrate that SptA functions both extracellularly and intracellularly, and that  sptA  expression relies on the cooperative action of multiple  cis -regulatory elements, allowing SptA to exert its function properly at different growth stages in strain J7-2.

frontiers in microbiology

Halolysin SptA, a Serine Protease, Contributes to Growth-Phase Transition of Haloarchaeon Natrinema sp. J7-2, and Its Expression Involves Cooperative Action of Multiple Cis-Regulatory Elements