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We studied the bacterial community in Rambla Salada in three different sampling sites and in three different seasons and the effect of salinity, oxygen, and pH. All sites samples had high diversity and richness (Rr > 30). The diversity indexes and the analysis of dendrograms obtained by DGGE fingerprint after applying Pearson's and Dice's coefficient showed a strong influence of sampling season. The Pareto-Lorenz (PL) curves and  Fo  analysis indicated that the microbial communities were balanced and despite the changing environmental conditions, they can preserve their functionality. The main phyla detected by DGGE were  Bacteroidetes  (39.73%),  Proteobacteria  (28.43%),  Firmicutes  (8.23%), and  Cyanobacteria  (5.14%). The majority of the sequences corresponding to uncultured bacteria belonged to  Bacteroidetes  phylum. Within  Proteobacteria , the main genera detected were  Halothiobacillus  and  Roseovarius . The environmental factors which influenced the community in a higher degree were the salinity and oxygen. The bacteria belonging to  Bacteroidetes  and  Proteobacteria  were positively influenced by salinity. Nevertheless, bacteria related to  Alpha-  and  Betaproteobacteria  classes and phylum  Firmicutes  showed a positive correlation with oxygen and pH but negative with salinity. The phylum  Cyanobacteria  were less influenced by the environmental variables. The bacterial community composition of Rambla Salada was also studied by dilution-to-extinction technique. Using this method, 354 microorganisms were isolated. The 16S sequences of 61 isolates showed that the diversity was very different to those obtained by DGGE and with those obtained previously by using classic culture techniques. The taxa identified by dilution-to-extinction were  Proteobacteria  (81.92%),  Firmicutes  (11.30%),  Actinobacteria  (4.52%), and  Bacteroidetes  (2.26%) phyla with  Gammaproteobacteria  as predominant class (65.7%). The main genera were:  Marinobacter  (38.85%),  Halomonas  (20.2%), and  Bacillus  (11.2%). Nine of the 61 identified bacteria showed less than 97% sequence identity with validly described species and may well represent new taxa. The number of bacteria in different samples, locations, and seasons were calculated by CARD-FISH, ranging from 54.3 to 78.9% of the total prokaryotic population. In conclusion, the dilution-to-extinction technique could be a complementary method to classical culture based method, but neither gets to cultivate the major taxa detected by DGGE. The bacterial community was influenced significantly by the physico-chemical parameters (specially the salinity and oxygen), the location and the season of sampling.

Study of Bacterial Community Composition and Correlation of Environmental Variables in Rambla Salada, a Hypersaline Environment in South-Eastern Spain