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Potato is prone to many drastic diseases like potato common scab (CS). As no highly effective methods exist for managing CS, this study explored the possibility of using biological control. Ten bacterial strains were isolated from CS-infected potato tubers from four different locations of Punjab, Pakistan, and identified based on biochemical and molecular analysis. Analysis of 16s rDNA sequences amplified by PCR revealed the isolated bacterial strains to be  Streptomyces scabies, S. turgidiscabies  and  S. stelliscabiei . Pathogenic islands were also confirmed among the isolates after identification of  txtAB, nec1 , and  tomA  genes with PCR amplification. One strain isolated from soil was antagonistic to the pathogenic  Streptomyces  spp., and determined to be  Streptomyces  A1RT on the basis of 16s rRNA sequencing. A methanolic extract of  Streptomyces  A1RT contained Isatropolone C, which was purified and structurally determined by  1 H- and  13 C-NMR,  1 H/ 1 H-COSY, HMQC, and HMBC techniques.  Streptomyces  A1RT also produced the plant growth hormone indole-3-acetic acid (IAA) with a titer of 26 μg ml -1  as confirmed by spectrophotometry and HPLC. In a greenhouse assay, disease severity index was established from 0 to 500. Average disease severity indexes were recorded as 63, 130.5, and 78 for  Streptomyces scabies, S. turgidiscabies  and  S. stelliscabiei , respectively. When  Streptomyces  A1RT was applied in soil that contained one of these pathogenic isolates, the average disease severity indexes were significantly ( P  < 0.05) reduced to 11.1, 5.6 and 8.4, respectively. A significant increase in tuber weight and shoot development was also observed with the tubers treated with  Streptomyces  A1RT. The use of the plant growth-promoting  Streptomyces  A1RT against potato CS thus provides an alternative strategy to control the disease without affecting environmental, plants, animals and human health.

Biological Control of Potato Common Scab With Rare Isatropolone C Compound Produced by Plant Growth Promoting Streptomyces A1RT