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A promising anti- Candida  activity of  Buchenavia tomentosa  extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a  B. tomentosa  extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100°C) and it was the most effective one. Gallic acid (GA) was identified by electrospray ionization mass spectrometry (ESI–MS) and also chosen to perform antifungal tests due to its promising activity on  Candida albicans . Minimal inhibitory and fungicidal concentrations (MIC and MFC) were determined by broth microdilution technique. The effect on virulence factors of  C. albicans  was evaluated, and the cytotoxicity was determined. MIC 50  and MIC 90  values were both equal to 0.625 mg ml -1  for AF and 2.5 and 5 mg ml -1 , respectively, for GA. AF and GA showed ability to inhibit  C. albicans  adherence and to disrupt 48 h-biofilm. AF and GA were effective in reducing the formation of hyphae of  C. albicans  SC5314. AF and GA decreased adherence of  C. albicans  to oral epithelial cells. AF and GA showed slight to moderate toxicity to Vero cells. This result suggests further studies for topic use of these compounds. AF, which contains a combination of several molecules, presented greater potential of antimicrobial activity than GA, with lower values of MIC and lower cytoxicity.

Effects of Acetone Fraction From Buchenavia tomentosa Aqueous Extract and Gallic Acid on Candida albicans Biofilms and Virulence Factors