English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Pigs are one of the most important economic livestock. Gut microbiota is not only critical to the health but also the production efficiency of pigs. Manipulating gut microbiota relies on the full view of gut microbiome and the understanding of drive forces shaping microbial communities. 16s rDNA sequencing was used to profile microbiota along the longitudinal and radical axes to obtain the topographical map of microbiome in different intestinal compartments in young pigs. Alpha and beta-diversities revealed distinct differences in microbial compositions between the distal ileum and cecum and colon, as well as between the lumen and mucosa.  Firmicutes  and  Proteobacteria  dominated in the ileum, constituting 95 and 80% of the luminal and mucosa-attached microbiome. Transitioning from the small intestine to the large intestine, luminal  Bacteroidetes  increased from 1.69 to 45.98% in the cecum and 40.09% in the colon, while mucosal  Bacteroidetes  raised from 9 to 35.36% and 27.96%. Concurrently, luminal  Firmicutes  and  Proteobacteria  and mucosal-attached  Proteobacteria  remarkably decreased. By co-occurrence network analyses,  Prevotellaceae, Ruminococcaceae, Lachnospiraceae  and  Veillonellaceae  were recognized as the central nodes of luminal microbial network, and  Prevotellaceae  and  Enterobacteriaceae, Caulobacteraceae, Enterococcaceae, Xanthomonadaceae, Pseudomonadaceae  were identified as mucosal central nodes. Co-abundance was uncovered among  Prevotellaceae, Lachnospiraceae , and  Veillonellaceae  in the luminal and mucosal microbiome, while opportunistic pathogens from γ- Proteobacteria  in the mucosa. Strong co-exclusion was shown between  Enterobacteriaceae  with  Prevotellaceae -centered microbial groups in the lumen. Redundancy analysis found bile acids and short chain fatty acids explained 37.1 and 41% of variations in the luminal microbial composition, respectively. Primary bile acid, taurine- and glycine- conjugated bile acids were positively correlated with  Lactobacillaceae, Enterobacteriaceae, Clostridiaceae_1, Peptostreptococcaceae , whereas secondary bile acids, acetate, propionate, butyrate, and valerate were positively correlated with  Prevotellaceae, Acidaminococcaceae, Ruminococcaceae, Lachnospiraceae, Desulfovibronaceae, Veillonellaceae . Functional analyses demonstrated that  Prevotella, Veillonellaceae, Lachnospiraceae , and  Ruminococcaceae  were positively correlated with gene functions related to amino acids, energy, cofactors and vitamins metabolism, which are indispensable for the hosts. These results suggested site specific colonization and co-occurrence of swine gut microbiome closely relate to the microenvironment in each niche. Interactions of core gut microbiome greatly contributed to metabolism and/or immunity in the swine intestine.

Spatial Heterogeneity and Co-occurrence of Mucosal and Luminal Microbiome across Swine Intestinal Tract