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Survival of photosynthetic cyanobacteria is challenged by environmental contaminations like heavy metals. Among them, deciphering the regulatory mechanisms for cadmium (Cd) in cyanobacteria would facilitate the construction of Cd-resistant strains. In this study, the DNA-Affinity-Purified-chromatin immunoprecipitation assay was employed to identify the direct targets of Sll0649, which was a Cd 2+ -related response regulator identified in our previous work in model cyanobacteria  Synechocystis  sp. PCC 6803. As a result, the promoter region of  slr0946  encoding the arsenate reductase was enriched fourfolds by quantitative real time PCR analysis. Further, deletion of  slr0946  led to a sensitive phenotype to Cd 2+  stress compared with the wild type (WT) and the sensitive phenotype of Δ slr0946  could be rescued by complementation assay via introducing  slr0946  back into Δ slr0946 . Finally, individually overexpression of  slr0946  as well as two Cd 2+ -related genes identified priviously (i.e.,  sll1598  and  slr0798 ) in WT could significantly improve the tolerance of  Synechocystis  sp. PCC 6803 to Cd 2+ . This study provided a better understanding of the tolerance mechanism to Cd 2+  in cyanobacteria and also feasible strategies for tolerance modifications to heavy metals in the future.

Identification of a New Target slr0946 of the Response Regulator Sll0649 Involving Cadmium Tolerance in Synechocystis sp. PCC 6803