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Human breast milk is recognized as one of multiple important sources of commensal bacteria for infant gut. Previous studies searched for the bacterial strains shared between breast milk and infant feces by isolating bacteria and performing strain-level bacterial genotyping, but only limited number of milk bacteria were identified to colonize infant gut, including bacteria from  Bifidobacterium ,  Staphylococcus ,  Lactobacillus , and  Escherichia / Shigella . Here, to identify the breast milk bacteria capable of colonizing gut without the interference of bacteria of origins other than the milk or the necessity to analyze infant feces, normal chow-fed germ-free mice were orally inoculated with the breast milk collected from a mother 2 days after vaginal delivery. According to 16S rRNA gene-based denaturant gradient gel electrophoresis and Illumina sequencing, bacteria at >1% abundance in the milk inoculum were only  Streptococcus  (56.0%) and  Staphylococcus  (37.4%), but in the feces of recipient mice were  Streptococcus  (80.3 ± 2.3%),  Corynebacterium  (10.0 ± 2.6 %),  Staphylococcus  (7.6 ± 1.6%), and  Propionibacterium  (2.1 ± 0.5%) that were previously shown as dominant bacterial genera in the meconium of C-section-delivered human babies; the abundance of anaerobic gut-associated bacteria,  Faecalibacterium ,  Prevotella ,  Roseburia ,  Ruminococcus , and  Bacteroides , was 0.01–1% in the milk inoculum and 0.003–0.01% in mouse feces; the abundance of  Bifidobacterium  spp. was below the detection limit of Illumina sequencing in the milk but at 0.003–0.01% in mouse feces. The human breast milk microbiota-associated mouse model may be used to identify additional breast milk bacteria that potentially colonize infant gut.

Development of Human Breast Milk Microbiota-Associated Mice as a Method to Identify Breast Milk Bacteria Capable of Colonizing Gut