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It is known that some bacteria, especially members of the family  Rhizobiaceae , have multiple  N -acyl homoserine lactones (AHL) synthase genes and produce multiple AHL signals. However, how bacteria selectively utilize these multiple genes and signals to cope with changing environments is poorly understood.  Ensifer adhaerens  is an important microorganism in terms of biotechnology, ecology and evolutionary. In this study, we investigated the AHL-based QS system of  E. adhaerens  X097 and its response to different lifestyles or nutrients. Draft genome sequence data indicated that X097 harbored three distinct AHL synthase genes ( ensI1, 2, 3 ) and seven  luxR  homologs, which was different from other  E. adhaerens  strains.  In vitro  expression indicated that plasmid-encoded  ensI1  and  ensI2  directed production of multiple AHLs, while chromosome-encoded  ensI3  only directed production of C14-HSL. Predicted three dimensional structure of EnsI3 was quite different from that of EnsI1 and EnsI2. X097 produced different AHL profiles in Luria-Bertani (LB) and NFB medium, under biofilm and planktonic lifestyle, respectively. Notably, expression of  ensI1  and  ensI2  but not  ensI3  is hypersensitive to different lifestyles and nutrients. The hypersensitive response of plasmid-encoded AHL synthase genes to different culture conditions may shed a light on the phylogenetic development of AHL synthase genes in  Rhizobiaceae  family.

Hypersensitive Response of Plasmid-Encoded AHL Synthase Gene to Lifestyle and Nutrient by Ensifer adhaerens X097