English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Thermoactinospora rubra  YIM 77501 T  is an aerobic, Gram-positive, spore-forming and cellulose degrading thermophilic actinomycete isolated from a sandy soil sample of a volcano. Its growth temperature range is 28–60°C. The genomic sequence of this strain revealed that there are 27 cellulase genes belonging to six glycoside hydrolase families. To understand the strategy that this strain uses to utilize carbon sources such as cellulose at different temperatures, comparative transcriptomics analysis of  T. rubra  YIM 77501 T  was performed by growing it with cellulose (CMC) and without cellulose (replaced with glucose) at 30, 40, and 50°C, respectively. Transcriptomic analyses showed four cellulase genes ( TrBG2, TrBG3, TrBG4 , and  ThrCel6B ) were up-regulated at 30, 40, and 50°C. The rate of gene expression of  TrBG2, TrBG3, TrBG4 , and  ThrCel6B  were 50°C > 30°C > 40°C. One cellulase gene ( TrBG1 ) and two cellulase genes ( TrBG5  and  ThrCel6A ) were up-regulated only at 30 and 50°C, respectively. These up-regulated cellulase genes were cloned and expressed in  Escherichia coli . The enzymatic properties of up-regulated cellulases showed a variety of responses to temperature. Special up-regulated cellulases  TrBG1  and  ThrCel6A  displayed temperature acclimation for each growth condition. These expression patterns revealed that a hybrid strategy was used by  T. rubra  to utilize carbon sources at different temperatures. This study provides genomic, transcriptomics, and experimental data useful for understanding how microorganisms respond to environmental changes and their application in enhancing cellulose hydrolysis for animal feed and bioenergy production.

The Hybrid Strategy of Thermoactinospora rubra YIM 77501T for Utilizing Cellulose as a Carbon Source at Different Temperatures