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JadL was identified as a Major Facilitator Superfamily (MFS) transporter (T.C. 2.A.1) through sequence homology. The protein is encoded by  jadL , situated within the jadomycin biosynthetic gene cluster. JadL has, therefore, been assigned a putative role in host defense by exporting its probable substrates, the jadomycins, a family of secondary metabolites produced by  Streptomyces venezuelae  ISP5230. Herein, we evaluate this assumption through the construction and analysis of a  jadL  disrupted mutant,  S. venezuelae  VS678  ( Δ jadL::aac(3)IV) . Quantitative determination of jadomycin production with the  jadL  disrupted mutant did not show a significant decrease in production in comparison to the wildtype strain, as determined by HPLC and by tandem mass spectrometry. These results suggest that efflux of jadomycin occurs upon disruption of  jadL , or that JadL is not involved in jadomycin efflux. Potentially, other transporters within  S. venezuelae  ISP5230 may adopt this role upon inactivation of JadL to export jadomycins.

frontiers in microbiology

Streptomyces venezuelae ISP5230 Maintains Excretion of Jadomycin upon Disruption of the MFS Transporter JadL Located within the Natural Product Biosynthetic Gene Cluster