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Background:  The emergence and spread of antimicrobial resistance has become a major global public health concern. A component element of this is the spread of the plasmid-encoded extended-spectrum b-lactamase (ESBL) genes, conferring resistance to third-generation cephalosporins. The purpose of this study was to investigate the molecular characteristics of ESBL-encoding genes identified in  Escherichia coli  cultured from diarrheic patients in China from 2013 to 2014.   Materials and Methods:  A total of 51  E. coli  were confirmed as ESBL producers by double-disk synergy testing of 912  E. coli  isolates studied. Polymerase chain reaction (PCR) and DNA sequencing were performed to identify the corresponding ESBL genes. Susceptibility testing was tested by the disk diffusion method. Plasmids were typed by PCR-based replicon typing and their sizes were determined by S1-nuclease pulsed-field gel electrophoresis. Multi-locus sequence typing (MLST) and phylogrouping were also performed. Broth mating assays were carried out for all isolates to determine whether the ESBL marker could be transferred by conjugation.   Results:  Of the 51 ESBL-positive isolates identified, bla CTX-M , bla TEM , bla OXA , and bla SHV  were detected in 51, 26, 3, 1 of these isolates, respectively. Sequencing revealed that 7 bla CTX-M  subtypes were detected, with bla CTX-M-14  being the most common, followed by bla CTX-M-79  and bla CTX-M-28 . Of the 26 TEM-positive isolates identified, all of these were bla TEM-1  genotypes. All isolates contained one to three large plasmids and 10 replicon types were detected. Of these, IncFrep ( n  = 50), IncK/B ( n  = 31), IncFIB ( n  = 26), IncB/O ( n  = 14), and IncI1-Ir ( n  = 8) replicon types were the predominating incompatibility groups. Twenty-six isolates demonstrated the ability to transfer their cefotaxime resistance marker at high transfer rates. MLST typing identified 31 sequence types and phylogenetic grouping showed that 12 of the 51 donor strains belonged to phylogroup B2.   Conclusion:  This study highlights the diversity of the ESBL producing  E. coli  and also the diversity of ESBL genes and plasmids carrying these genes in China, which poses a threat to public health.

Prevalence and Molecular Characteristics of Extended-Spectrum β-Lactamase Genes in Escherichia coli Isolated from Diarrheic Patients in China