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Bacterial surface proteins can be good vaccine candidates. In the present study, we used polyclonal antibodies purified with intact  Erysipelothrix rhusiopthiae  to screen phage-displayed random dodecapeptide and loop-constrained heptapeptide libraries, which led to the identification of mimotopes. Homology search of the mimotope sequences against  E. rhusiopthiae -encoded ORF sequences revealed 14 new antigens that may localize on the surface of  E. rhusiopthiae . When these putative surface proteins were used to immunize mice, 9/11 antigens induced protective immunity. Thus, we have demonstrated that a combination of using the whole bacterial cells to purify antibodies and using the phage-displayed peptide libraries to determine the antigen specificities of the antibodies can lead to the discovery of novel bacterial surface antigens. This can be a general approach for identifying surface antigens for other bacterial species.

Identification of Bacterial Surface Antigens by Screening Peptide Phage Libraries Using Whole Bacteria Cell-Purified Antisera