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This study was undertaken to characterize a lactic acid bacterium 4I1, isolated from the freshwater fish,  Zacco koreanus . Morphological, biochemical, and molecular characterization of 4I1 revealed it to be  Pediococcus pentosaceus  4I1. The cell free supernatant (CFS) of  P. pentosaceus  4I1 exhibited significant ( p  < 0.05) antibacterial effects (inhibition zone diameters: 16.5–20.4 mm) against tested foodborne pathogenic bacteria with MIC and MBC values of 250–500 and 500–1,000 μg/mL, respectively. Further, antibacterial action of CFS of  P. pentosaceus  4I1 against two selected bacteria  Staphylococcus aureus  KCTC-1621 and  Escherichia coli  O157:H7 was determined in subsequent assays. The CFS of  P. pentosaceus  4I1 revealed its antibacterial action against  S. aureus  KCTC-1621 and  E. coli  O157:H7 on membrane integrity as confirmed by a reduction in cell viability, increased potassium ion release (900 and 800 mmol/L), reduced absorption at 260-nm (3.99 and 3.77 OD), and increased relative electrical conductivity (9.9 and 9.7%), respectively. Gas chromatography–mass spectrometry (GC–MS) analysis of the CFS of  P. pentosaceus  4I1 resulted in the identification of seven major compounds, which included amino acids, fatty acids and organic acids. Scanning electron microscopic-based morphological analysis further confirmed the antibacterial effect of CFS of  P. pentosaceus  4I1 against  S. aureus  KCTC-1621 and  E. coli  O157:H7. In addition, the CFS of  P. Pentosaceus  4I1 displayed potent inhibitory effects on biofilms formation by  S. aureus  KCTC-1621 and  E. coli  O157:H7. The study indicates the CFS of  P. pentosaceus  4I1 offers an alternative means of controlling foodborne pathogens.

Characterization and Antibacterial Potential of Lactic Acid Bacterium Pediococcus pentosaceus 4I1 Isolated from Freshwater Fish Zacco koreanus