English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The 2-alkyl-3-hydroxy-4(1 H )-quinolone 2,4-dioxygenase HodC was previously described to cleave the  Pseudomonas  quinolone signal, PQS, which is exclusively used in the complex quorum sensing (QS) system of  Pseudomonas aeruginosa , an opportunistic pathogen employing QS to regulate virulence and biofilm development. Degradation of PQS by exogenous addition of HodC to planktonic cells of  P. aeruginosa  attenuated production of virulence factors, and reduced virulence  in planta . However, proteolytic cleavage reduced the efficacy of HodC. Here, we identified the secreted protease LasB of  P. aeruginosa  to be responsible for HodC degradation. In static biofilms of the  P. aeruginosa  PA14  lasB ::Tn mutant, the catalytic activity of HodC led to an increase in viable biomass in newly formed but also in established biofilms, and reduced the expression of genes involved in iron metabolism and siderophore production, such as  pvdS, pvdL, pvdA , and  pvdQ . This is likely due to an increase in the levels of bioavailable iron by degradation of PQS, which is able to sequester iron from the surrounding environment. Thus, HodC, despite its ability to quench the production of virulence factors, is contraindicated for combating  P. aeruginosa  biofilms.

frontiers in microbiology

Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS) Promotes Biofilm Formation of Pseudomonas aeruginosa by Increasing Iron Availability