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Salmonella enterica  serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional  Salmonella  serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step polymerase chain reaction (PCR) method to identify  S.  Pullorum/Gallinarum. The PCR-based assay focuses on  flhB , which shows a deficient region only in  S.  Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identify  S.  Pullorum/Gallinarum from 27 different  Salmonella  serovars and eight non- Salmonella  pathogens. The minimum limit of DNA and the lowest number of cells of  S.  Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis of  Salmonella  strains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence of  S.  Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations.

One-Step PCR Detection of Salmonella Pullorum/Gallinarum Using a Novel Target: The Flagellar Biosynthesis Gene flhB