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The social amoeba  Dictyostelium discoideum  is a well-established model organism to study the interaction between bacteria and phagocytes. In contrast, research using  D. discoideum  as a host model for fungi is rare. We describe a comprehensive study, which uses  D. discoideum  as a host model system to investigate the interaction with apathogenic ( Saccharomyces cerevisiae ) and pathogenic ( Candida  sp.) yeast. We show that  Dictyostelium  can be co-cultivated with yeasts on solid media, offering a convenient test to study the interaction between fungi and phagocytes. We demonstrate that a number of  D. discoideum  mutants increase ( atg1  − ,  kil1  − ,  kil2  − ) or decrease ( atg6  − ) the ability of the amoebae to predate yeast cells. On the yeast side, growth characteristics, reduced phagocytosis rate, as well as known virulence factors of  C. albicans  ( EFG1, CPH1, HGC1, ICL1 ) contribute to the resistance of yeast cells against predation by the amoebae. Investigating haploid  C. albicans  strains, we suggest using the amoebae plate test for screening purposes after random mutagenesis. Finally, we discuss the potential of our adapted amoebae plate test to use  D. discoideum  for risk assessment of yeast strains.

Dictyostelium discoideum as a Novel Host System to Study the Interaction between Phagocytes and Yeasts