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Here we introduce a novel strategy for turbidimetric monitoring of bacterial growth in liquid culture. The instrumentation comprises a light source, a customized 3D printed culture tube holder and a miniaturized spectrophotometer, connected through optical cables. Due to its small footprint and the possibility to operate with external light, bacterial growth was directly monitored from culture tubes in a simple and versatile fashion. This new portable measurement technique was used to monitor the growth of facultative ( Escherichia coli  ATCC/25922, and  Staphylococcus aureus  ATCC/29213) and strictly ( Butyrivibrio fibrisolvens  JW11,  Butyrivibrio proteoclasticus  P18, and  Propionibacterium acnes  DSMZ 1897) anaerobic bacteria. For  E. coli  and  S. aureus , the growth rates calculated from normalized optical density values were compared with those ones obtained using a benchtop spectrophotometer without significant differences ( P  = 0.256). For the strictly anaerobic species, a high precision (relative standard deviation < 3.5%) was observed between replicates up to 48 h. Regarding its potential for customization, this manifold could accommodate further developments for customized turbidimetric monitoring, such as the use of light-emitting diodes as a light source or flow cells.

frontiers in microbiology

Simple and Versatile Turbidimetric Monitoring of Bacterial Growth in Liquid Cultures Using a Customized 3D Printed Culture Tube Holder and a Miniaturized Spectrophotometer: Application to Facultative and Strictly Anaerobic Bacteria