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LexA is a well-established transcriptional repressor of SOS genes induced by DNA damage in  Escherichia coli  and other bacterial species. However, LexA in the cyanobacterium  Synechocystis  sp. PCC 6803 has been suggested not to be involved in SOS response. In this study, we performed RNA-seq analysis of the wild-type strain and the  lexA -disrupted mutant to obtain the comprehensive view of LexA-regulated genes in  Synechocystis . Disruption of  lexA  positively or negatively affected expression of genes related to various cellular functions such as phototactic motility, accumulation of the major compatible solute glucosylglycerol and subunits of bidirectional hydrogenase, photosystem I, and phycobilisome complexes. We also observed increase in the expression level of genes related to iron and manganese uptake in the mutant at the later stage of cultivation. However, none of the genes related to DNA metabolism were affected by disruption of  lexA . DNA gel mobility shift assay using the recombinant LexA protein suggested that LexA binds to the upstream region of  pilA7, pilA9, ggpS , and  slr1670  to directly regulate their expression, but changes in the expression level of photosystem I genes by disruption of  lexA  is likely a secondary effect.

RNA-seq Profiling Reveals Novel Target Genes of LexA in the Cyanobacterium Synechocystis sp. PCC 6803