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Acinetobacter baumannii  is emerging as a serious nosocomial pathogen with multidrug resistance that has made it difficult to cure and development of efficacious treatment against this pathogen is direly needed. This has led to investigate vaccine approach to prevent and treat  A. baumannii  infections. In this work, an outer membrane putative pilus assembly protein, FilF, was predicted as vaccine candidate by  in silico  analysis of  A. baumannii  proteome and was found to be conserved among the  A. baumannii  strains. It was cloned and expressed in  E. coli  BL21(DE3) and purified by Ni-NTA chromatography. Immunization with FilF generated high antibody titer (>64,000) and provided 50% protection against a standardized lethal dose (10 8  CFU) of  A. baumannii  in murine pneumonia model. FilF immunization reduced the bacterial load in lungs by 2 and 4 log cycles, 12 and 24 h post infection as compared to adjuvant control; reduced the levels of pro-inflammatory cytokines TNF-α, IL-6, IL-33, IFN-γ, and IL-1β significantly and histology of lung tissue supported the data by showing considerably reduced damage and infiltration of neutrophils in lungs. These results demonstrate the  in vivo  validation of immunoprotective efficacy of a protein predicted as a vaccine candidate by  in silico  proteomic analysis and open the possibilities for exploration of a large array of uncharacterized proteins.

Immunoprotective Efficacy of Acinetobacter baumannii Outer Membrane Protein, FilF, Predicted In silico as a Potential Vaccine Candidate