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Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in  Saccharomyces cerevisiae . Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of  XylA  and endogenous  XKS1 . Additional overexpression of genes  RKI1, RPE1, TKL1 , and  TAL1  in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.

Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae