English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the MALDI Bruker Biotyper system (microflex LT; Bruker Daltonik GmbH, Bremen, Germany), on the identification of 50 isolates of clinically encountered molds, including  Penicillium marneffei  ( n  = 28),  Paecilomyces  species ( n  = 12),  Fusarium solani  ( n  = 6),  Rhizopus  species ( n  = 3), and  Pseudallescheria boydii  ( n  = 1). The isolates were identified to species levels by sequence analysis of the internal transcribed spacer (ITS) regions using primers ITS1 and ITS4. None of the 28 genetically well characterized isolates of  P. marneffei  were identified as  P. marneffei  by MALDI-TOF MS, because  P. marneffei  was not present in either Bruker general library (DB 5627) or Bruker filamentous fungi library V1.0. However, the rate of accurate identification as  P. marneffei  (score value ≥ 2.000) was 85.7% based on newly created database from one  P. marneffei  strain (NTUH-3370) by MALDI Biotyper system. Sequencing analysis of these 22 non- P. marneffei  isolates of molds revealed seven  Paecilomyces variotii , six  F. solani , four  Paecilomyces lilacinus , and one each of  Paecilomyces sinensis, Rhizopus arrhizus, R. oryzae, R. microspores , and  P. boydii . Although all the seven  P. variotii  isolates, four of the six  F. solani , two of the four  P. lilacinus , and two of the three isolates of  Rhizopus  species, and the  P. boydii  isolate had concordant identification results between MALDI-TOF MS and sequencing analysis, the score values of these isolates were all of <1.700. This study indicated that the MALDI Bruker Biotyper is ineffective for identifying  P. marneffei  and other unusual molds because of the current database limitations. Therefore, it is necessary to continuously update the MALDI-TOF MS databases.

frontiers in microbiology

Evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Bruker Biotyper for identification of Penicillium marneffei, Paecilomyces species, Fusarium solani, Rhizopus species, and Pseudallescheria boydii