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The  s -triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors:  E. coli  SM003 for cyanuric acid detection and  E. coli  SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for  E. coli  SM003 ranges from 7.83 μM to 2.89 mM, and for  E. coli  SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by  E. coli  SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by  E. coli  SM004 is only applicable for high concentrations of contaminants.

Development of a bacterial bioassay for atrazine and cyanuric acid detection