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Intracellular life of  Helicobacter pylori  inside  Candida  yeast vacuole describes the establishment of  H. pylori  in yeast as a pre-adaptation to life in human epithelial cells. IgY-Hp conjugated with fluorescein isothiocyanate (FITC) has been previously used for identification and localization of  H. pylori  inside the yeast vacuole. Here we examined whether FITC-IgY-Hp internalization into yeast follows the endocytosis pathway in yeast. Fluorescent microscopy was used to examine the entry of FITC-IgY-Hp into  Candida  yeast cells at different time intervals. The effect of low temperature, H 2 O 2  or acetic acid on the internalization of labeled antibody was also examined. FITC-IgY-Hp internalization initiated within 0–5 min in 5–10% of yeast cells, increased to 20–40% after 30 min–1 h and reached >70% before 2 h. FITC-IgY-Hp traversed the pores of  Candida  yeast cell wall and reached the vacuole where it bound with  H. pylori  antigens. Internalization of FITC-IgY-Hp was inhibited by low temperature, H 2 O 2  or acetic acid. It was concluded that internalization of FITC-IgY-Hp into yeast cell is a vital phenomenon and follows the endocytosis pathway. Furthermore, it was proposed that FITC-IgY-Hp internalization could be recruited for localization and identification of  H. pylori  inside the vacuole of  Candida  yeast.

Endocytotic uptake of FITC-labeled anti-H. pylori egg yolk immunoglobulin Y in Candida yeast for detection of intracellular H. pylori