English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The purpose of this study was to examine the occurrence of fosfomycin-resistant  Escherichia coli  from chickens and to characterize the plasmids carrying  fosA3 . A total of 661  E. coli  isolates of chicken origin collected from 2009 to 2011 were screened for plasmid-mediated fosfomycin resistance determinants by PCR. Plasmids were characterized using PCR-based replicon typing, plasmid multilocus sequence typing, and restriction fragment length polymorphisms. Associated addiction systems and resistance genes were identified by PCR. PCR-mapping was used for analysis of the genetic context of  fosA3 . Fosfomycin resistance was detected in 58 isolates that also carried the  fosA3  gene. Fifty-seven, 17, and 52 FosA3-producers also harbored  bla  CTX−M ,  rmtB , and  floR  genes, respectively. Most of the 58  fosA3 -carrying isolates were clonally unrelated, and all  fosA3  genes were located on plasmids belonged to F33:A-:B- ( n  = 18), IncN-F33:A-:B- ( n  = 7), IncHI2/ST3 ( n  = 10), IncI1/ST71 ( n  = 3), IncI1/ST108 ( n  = 3), and others. The genetic structures, IS 26-ISEcp1 - bla  CTX−M−55 - orf477 - bla  TEM-1 -IS 26 - fosA3 -1758bp-IS 26  and IS Ecp1 - bla  CTX−M−65 -IS 903 -iroN-IS 26 - fosA3 -536bp-IS 26  were located on highly similar F33:A-:B- plasmids. In addition,  bla  CTX−M−14 - fosA3 -IS 26  was frequently present on similar IncHI2/ST3 plasmids. IncFII plasmids had a significantly higher frequency of addiction systems (mean 3.5) than other plasmids. Our results showed a surprisingly high prevalence of  fosA3  gene in  E. coli  isolates recovered from chicken in China. The spread of  fosA3  can be attributed to horizontal dissemination of several epidemic plasmids, especially F33:A-:B- plasmids. Since coselection by other antimicrobials is the major driving force for the diffusion of the  fosA3  gene, a strict antibiotic use policy is urgently needed in China.

frontiers in microbiology

F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTXâˆ’Mâˆ’55/âˆ’14/âˆ’65 in Escherichia coli from chickens in China