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C-type allatostatins (C-type ASTs) are a family of structurally related neuropeptides found in a wide range of insects and crustaceans. To date, the C-type allatostatin receptor in crustaceans has not been deorphaned, and little is known about its physiological functions. In this study, we aimed to functionally define a C-type ASTs receptor in the mud crab,  Scylla paramamosian . We showed that C-type ASTs receptor can be activated by  Scypa AST-C peptide in a dose-independent manner and by  Scypa AST-CCC peptide in a dose-dependent manner with an IC 50  value of 6.683 nM. Subsequently,  in vivo  and  in vitro  experiments were performed to investigate the potential roles of  Scypa AST-C and  Scypa AST-CCC peptides in the regulation of ecdysone (20E) and methyl farnesoate (MF) biosynthesis. The results indicated that  Scypa AST-C inhibited biosynthesis of 20E in the Y-organ, whereas  Scypa AST-CCC had no effect on the production of 20E. In addition, qRT-PCR showed that both  Scypa AST-C and  Scypa AST-CCC significantly decreased the level of expression of the MF biosynthetic enzyme gene in the mandibular organ, suggesting that the two neuropeptides have a negative effect on the MF biosynthesis in mandibular organs. In conclusion, this study provided new insight into the physiological roles of AST-C in inhibiting ecdysone biosynthesis. Furthermore, it was revealed that AST-C family peptides might inhibit MF biosynthesis in crustaceans.

A Possible Role of Allatostatin C in Inhibiting Ecdysone Biosynthesis Revealed in the Mud Crab Scylla paramamosain