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Fc receptor (FcR) is an important opsonin receptor on the surface of immune cells, playing an important role in antibody-dependent cell-mediated immunity. Our previous work found that the FcR of flounder showed a marked expression response in phagocytizing IgM +  B cell, which suggested that FcR might participate in regulating Ig-opsonized phagocytosis. In this paper, in order to elucidate the potential role of FcR in mediating phagocytosis of IgM +  B cell, flounder anti- E. tarda  serum was prepared and complement-inactivated for the use of  E. tarda  opsonization, and the sera of healthy flounder were used as control. Flow cytometric analysis showed that the phagocytosis rates of antiserum-opsonized  E. tarda  in peripheral blood mIgM +  B lymphocytes were significantly higher than the control group, and higher phagocytosis rates of mIgM +  B lymphocyte could be detected with an increasing incubation time ranging from 1 to 5 h. The phagocytosis rates of antiserum-opsonized  E. tarda  by mIgM +  B lymphocyte for an incubation time of 1, 3 or 5 h were 51.1, 63.0, and 77.5% respectively, which were significantly higher than the phagocytosis rates in the control groups with 40.2, 50.9, and 63.8%, respectively. While the Fc fragment of IgM on the surface of opsonized  E. tarda  was blocked by rabbit anti-flounder IgM polyclonal antibodies, phagocytosis rates of mIgM +  B lymphocyte decreased significantly compared with the unblocked group. Moreover, the proportion of mIgM +  B lymphocytes with higher intracellular reactive oxygen species (ROS) levels rose to 32.1% from the control level of 23.0% after phagocytosis of antiserum-opsonized  E. tarda . FcγRII and Syk were found to be significantly upregulated, while FcγRIII was significantly downregulated in the mIgM +  B lymphocytes post phagocytosis. Furthermore, when FcγRII of mIgM +  B lymphocytes was blocked by the prepared antibodies, their phagocytosis rate of antiserum-opsonized  E. tarda  was 39.0%, which was significantly lower than the unblocked group of 54.0%. These results demonstrate that FcR plays a critical role in mediating phagocytosis and bactericidal activity of mIgM +  B lymphocytes, which would facilitate an improved understanding of the regulatory roles of FcR in phagocytosis of teleost B lymphocytes.

Regulatory Role of Fc Receptor in mIgM+ B Lymphocyte Phagocytosis in Flounder (Paralichthys olivaceus)