English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Bid (BH3-interacting domain death agonist), a member of the Bcl-2 family, plays a crucial role in the initiation of apoptosis. Independent of its apoptotic function, Bid is also involved in the regulation of inflammation and innate immunity. However, the role of Bid during bacterial pathogen infection remains unclear. In the present study,  Bid  of zebrafish ( Dario rerio ) was cloned and its functions during  Edwardsiella ictaluri  infection were investigated. Zebrafish Bid enhances the apoptosis rate of  Epithelioma papulosum cyprini  (EPC) cells following  E. ictaluri  infection. Importantly,  in vitro  and  in vivo  bacterial invasion assays showed that overexpressed Bid could significantly inhibit the invasion and proliferation of  E. ictaluri . Real-time qPCR analysis revealed that p53 gene expression was downregulated in embryos microinjected with Bid-FLAG. Further,  in vitro  and  in vivo  bacterial invasion assays showed that overexpressed p53 increased the invasion and proliferation of  E. ictaluri . Moreover, the invasion and proliferation of  E. ictaluri  were inhibited when co-overexpressing Bid and p53  in vivo  and  in vitro . Further, the numbers of  E. ictaluri  in larvae treated with Z-IETD-FMK (caspase-8 inhibitor) were higher than those of larvae without Z-IETD-FMK treatment, while the number of  E. ictaluri  in larvae microinjected with  bid -Flag decreased significantly, even if the larvae were treated in advance with Z-IETD-FMK. Collectively, our study demonstrated a novel antibacterial activity of fish Bid, providing evidence for understanding the function of apoptosis associated gene in pathogen infection.

Zebrafish BID Exerts an Antibacterial Role by Negatively Regulating p53, but in a Caspase-8-Independent Manner