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Extensive exploitation of titanium dioxide nanoparticles (TiO 2 NPs) augments rapid release into the marine environment. When in contact with the body fluids of marine invertebrates, TiO 2 NPs undergo a transformation and adhere various organic molecules that shape a complex protein corona prior to contacting cells and tissues. To elucidate the potential extracellular signals that may be involved in the particle recognition by immune cells of the sea urchin  Paracentrotus lividus , we investigated the behavior of TiO 2 NPs in contact with extracellular proteins  in vitro . Our findings indicate that TiO 2 NPs are able to interact with sea urchin proteins in both cell-free and cell-conditioned media. The two-dimensional proteome analysis of the protein corona bound to TiO 2 NP revealed that negatively charged proteins bound preferentially to the particles. The main constituents shaping the sea urchin cell-conditioned TiO 2 NP protein corona were proteins involved in cellular adhesion ( Pl -toposome,  Pl -galectin-8,  Pl -nectin) and cytoskeletal organization (actin and tubulin). Immune cells (phagocytes) aggregated TiO 2 NPs on the outer cell surface and within well-organized vesicles without eliciting harmful effects on the biological activities of the cells. Cells showed an active metabolism, no oxidative stress or caspase activation. These results provide a new level of understanding of the extracellular proteins involved in the immune-TiO 2 NP recognition and interaction  in vitro , confirming that primary immune cell cultures from  P. lividus  can be an optional model for swift and efficient immune-toxicological investigations.

Sea Urchin Extracellular Proteins Design a Complex Protein Corona on Titanium Dioxide Nanoparticle Surface Influencing Immune Cell Behavior