English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Vaccination is considered the most effective strategy for controlling tuberculosis (TB). The existing vaccine, the Bacille Calmette-Guérin (BCG), although partially protective, has a number of limitations. Therefore, there is a need for developing new TB vaccines and several strategies are currently exploited including the use of viral and bacterial delivery vectors. We have previously shown that  Lactobacillus plantarum  ( Lp ) producing Ag85B and ESAT-6 antigens fused to a dendritic cell-targeting peptide (referred to as  Lp _DC) induced specific immune responses in mice. Here, we analyzed the ability of two  Lp -based vaccines,  Lp _DC and  Lp _HBD (in which the DC-binding peptide was replaced by an HBD-domain directing the antigen to non-phagocytic cells) to activate antigen-presenting cells, induce specific immunity and protect mice from  Mycobacterium tuberculosis  infection. We tested two strategies: (i)  Lp  as BCG boosting vaccine (a heterologous regimen comprising parenteral BCG immunization followed by intranasal  Lp  boost), and (ii)  Lp  as primary vaccine (a homologous regimen including subcutaneous priming followed by intranasal boost). The results showed that both  Lp  constructs applied as a BCG boost induced specific cellular immunity, manifested in T cell proliferation, antigen-specific IFN-γ responses and multifunctional T cells phenotypes. More importantly, intranasal boost with  Lp _DC or  Lp _HBD enhanced protection offered by BCG, as shown by reduced  M. tuberculosis  counts in lungs. These findings suggest that  Lp  constructs could be developed as a potential mucosal vaccine platform against mycobacterial infections.

frontiers in immunology

Inactivated Lactobacillus plantarum Carrying a Surface-Displayed Ag85B-ESAT-6 Fusion Antigen as a Booster Vaccine Against Mycobacterium tuberculosis Infection