English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Type 1 conventional DCs (cDC1) excel in the cross-priming of CD8 +  T cells, which is crucial for orchestrating efficient immune responses against viruses or tumors. However, our understanding of their physiological functions and molecular regulation has been limited by the lack of proper mutant mouse models allowing their conditional genetic targeting. Because the  Xcr1  and  A530099j19rik  ( Karma/Gpr141b)  genes belong to the core transcriptomic fingerprint of mouse cDC1, we used them to engineer two novel Cre-driver lines, the  Xcr1   Cre   and  Karma   Cre   mice, by knocking in an IRES-Cre expression cassette into their 3′-UTR. We used genetic tracing to characterize the specificity and efficiency of these new models in several lymphoid and non-lymphoid tissues, and compared them to the  Clec9a   Cre   mouse model, which targets the immediate precursors of cDCs. Amongst the three Cre-driver mouse models examined, the  Xcr1   Cre   model was the most efficient and specific for the fate mapping of all cDC1, regardless of the tissues examined. The  Karma   Cre   model was rather specific for cDC1 when compared with the  Clec9a   Cre   mouse, but less efficient than the  Xcr1   Cre   model. Unexpectedly, the  Xcr1   Cre   model targeted a small fraction of CD4 +  T cells, and the  Karma   Cre   model a significant proportion of mast cells in the skin. Importantly, the targeting specificity of these two mouse models was not changed upon inflammation. A high frequency of germline recombination was observed solely in the  Xcr1   Cre   mouse model when both the  Cre  and the  floxed  alleles were brought by the same gamete irrespective of its gender.  Xcr1, Karma , and  Clec9a  being differentially expressed within the cDC1 population, the three CRE-driver lines examined showed distinct recombination patterns in cDC1 phenotypic subsets. This advances our understanding of cDC1 subset heterogeneity and the differentiation trajectory of these cells. Therefore, to the best of our knowledge, upon informed use, the  Xcr1   Cre   and  Karma   Cre   mouse models represent the best tools currently reported to specifically and faithfully target cDC1  in vivo , both at steady state and upon inflammation. Future use of these mutant mouse models will undoubtedly boost our understanding of the biology of cDC1.

frontiers in immunology

Novel Cre-Expressing Mouse Strains Permitting to Selectively Track and Edit Type 1 Conventional Dendritic Cells Facilitate Disentangling Their Complexity in vivo