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Neurokinin (NK) signaling is involved in various inflammatory processes. A common manifestation of systemic inflammation is fever, which is usually induced in animal models with the administration of bacterial lipopolysaccharide (LPS). A role for the NK1 receptor was shown in LPS-induced fever, but the underlying mechanisms of how the NK1 receptor contributes to febrile response, especially in the early phase, have remained unknown. We administered LPS (120 µg/kg, intraperitoneally) to mice with the  Tacr1  gene, i.e., the gene encoding the NK1 receptor, either present ( Tacr1 +/+  ) or absent ( Tacr1 −/−  ) and measured their thermoregulatory responses, serum cytokine levels, tissue cyclooxygenase-2 (COX-2) expression, and prostaglandin (PG) E 2  concentration. We found that the LPS-induced febrile response was attenuated in  Tacr1 −/−   compared to their  Tacr1 +/+   littermates starting from 40 min postinfusion. The febrigenic effect of intracerebroventricularly administered PGE 2  was not suppressed in the  Tacr1 −/−   mice. Serum concentration of pyrogenic cytokines did not differ between  Tacr1 −/−   and  Tacr1 +/+   at 40 min post-LPS infusion. Administration of LPS resulted in amplification of COX-2 mRNA expression in the lungs, liver, and brain of the mice, which was statistically indistinguishable between the genotypes. In contrast, the LPS-induced augmentation of COX-2 protein expression was attenuated in the lungs and tended to be suppressed in the liver of  Tacr1 −/−   mice compared with  Tacr1 +/+   mice. The  Tacr1 +/+   mice responded to LPS with a significant surge of PGE 2  production in the lungs, whereas  Tacr1 −/−   mice did not. In conclusion, the NK1 receptor is necessary for normal fever genesis. Our results suggest that the NK1 receptor contributes to the early phase of LPS-induced fever by enhancing COX-2 protein expression in the periphery. These findings advance the understanding of the crosstalk between NK signaling and the “cytokine-COX-2-prostaglandin E 2 ” axis in systemic inflammation, thereby open up the possibilities for new therapeutic approaches.

frontiers in immunology

The Neurokinin-1 Receptor Contributes to the Early Phase of Lipopolysaccharide-Induced Fever via Stimulation of Peripheral Cyclooxygenase-2 Protein Expression in Mice