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Previous studies have revealed that  SDHB  has potential functions in the male sexual differentiation and development in  M .  nipponense  through providing ATP. In this study, the functions of  Mn-SDHB  were further analyzed in depth using quantitative polymerase chain reaction (qPCR),  in situ  hybridization, western-blot, and RNA interference (RNAi), combined with the histological observations. The full-genome sequence of  Mn-SDHB  was 54,608 bp at Chromosome 34, including 7 introns and 6 exons. The full-length cDNA sequence of  Mn-SDHB  was 1,268 base pairs (bp) long with an open reading frame of 807 bp, encoding for 268 amino acids. The highest expression level of  Mn-SDHB  in different tissues was observed in the testis, and male prawns at post-larval developmental stage 25 during different developmental stages, indicating that  SDHB  was potentially involved in the male sexual development in  M .  nipponense .  In situ  hybridization and western-blot analysis indicated that  SDHB  plays essential roles in the testis development. The  in situ  hybridization analysis also implies the potential roles of  Mn-SDHB  in ovarian development. The expressions of  Mn-IAG  were decreased after  Mn-SDHB  dsRNA injection, indicating  SDHB  has the positive regulatory effects on  IAG  in  M .  nipponese . Thus,  SDHB  was involved in the mechanism of the male sexual development. The testis development was inhibited, and sperms were rarely observed after 10 days of  Mn-SDHB  dsRNA injection, indicating  SDHB  has positive effects on the male sexual development in  M .  nipponense . This study highlights the functions of  SDHB  in  M .  nipponense , which provide new insights for the future studies of the male sexual development in other crustacean species.

frontiers in genetics

Identification and Characterization of the Succinate Dehydrogenase Complex Iron Sulfur Subunit B Gene in the Oriental River Prawn, Macrobrachium nipponense