English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The genus  Burkholderia  contains large number of diverse species which include many clinically important organisms, phytopathogens, as well as environmental species. However, currently, there is a paucity of biochemical or molecular characteristics which can reliably distinguish different groups of  Burkholderia  species. We report here the results of detailed phylogenetic and comparative genomic analyses of 45 sequenced species of the genus  Burkholderia . In phylogenetic trees based upon concatenated sequences for 21 conserved proteins as well as 16S rRNA gene sequence based trees, members of the genus  Burkholderia  grouped into two major clades. Within these main clades a number of smaller clades including those corresponding to the clinically important  Burkholderia cepacia  complex (BCC) and the  Burkholderia pseudomallei  groups were also clearly distinguished. Our comparative analysis of protein sequences from  Burkholderia  spp. has identified 42 highly specific molecular markers in the form of conserved sequence indels (CSIs) that are uniquely found in a number of well-defined groups of  Burkholderia  spp. Six of these CSIs are specific for a group of  Burkholderia  spp. (referred to as Clade I in this work) which contains all clinically relevant members of the genus (viz. the BCC and the  B. pseudomallei  group) as well as the phytopathogenic  Burkholderia  spp. The second main clade (Clade II), which is composed of environmental  Burkholderia  species, is also distinguished by 2 identified CSIs that are specific for this group. Additionally, our work has also identified multiple CSIs that serve to clearly demarcate a number of smaller groups of  Burkholderia  spp. including 3 CSIs that are specific for the  B. cepacia  complex, 4 CSIs that are uniquely found in the  B. pseudomallei  group, 5 CSIs that are specific for the phytopathogenic  Burkholderia  spp. and 22 other CSI that distinguish two groups within Clade II. The described molecular markers provide highly specific means for the demarcation of different groups of  Burkholderia  spp. and they also offer novel and useful targets for the development of diagnostic assays for the clinically important members of the BCC or the  pseudomallei  groups. Based upon the results of phylogenetic analyses, the identified CSIs and the pathogenicity profile of  Burkholderia  species, we are proposing a division of the genus  Burkholderia  into two genera. In this new proposal, the emended genus  Burkholderia  will correspond to the Clade I and it will contain only the clinically relevant and phytopathogenic  Burkholderia  species. All other  Burkholderia  spp., which are primarily environmental, will be transferred to a new genus  Paraburkholderia  gen. nov.

frontiers in genetics

Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental species