English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Legumain , also called  aspartic endopeptidase  (AEP), is a member of the cysteine protease family and is involved in various physiological processes. In this study, we analyzed the characteristics of a novel  legumain-like  (named  Mn-Lel ) in the female oriental river prawn,  Macrobrachium nipponense , which is involved in ovary maturation. The  Mn-Lel  is 1,454 bp in length, including a 1,290-bp open reading frame that encodes 430 amino acids. qPCR analysis indicated that  Mn-Lel  is specifically highly expressed in the hepatopancreas and ovaries of female prawns. It is rarely expressed in embryogenesis, weakly expressed in early larval development stages, and then significantly increased after metamorphosis, which indicated that  Mn-Lel  is not a maternal gene and mainly plays a role in adults. During the different ovarian stages,  Mn-Lel  expression in the hepatopancreas had no obvious rules, while its expression in the ovaries had a significant peak in stage III.  In situ  hybridization studies revealed that  Mn-Lel  is localized in the oocyte of the ovary. Changes in the gonadosomatic index confirmed the inhibitory effects of  Mn-Lel  dsRNA on ovary maturation. These results suggest that  Mn-Lel  has a key role in promoting ovary maturation.

A Novel Legumain-Like Protease in Macrobrachium nipponense: Identification, Characterization, and Function Analysis in Ovary Maturation