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Cathepsin L  genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a  cathepsin L  (named  Mn-CL2 ) in the female oriental river prawn,  Macrobrachium nipponense  which was involved in ovary maturation. The  Mn-CL2  was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The  Mn-CL2  was classified into the  cathepsin L  group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that  Mn-CL2  was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages,  Mn-CL2  expression in the hepatopancreas and ovaries peaked before ovarian maturation.  In situ  hybridization studies revealed that  Mn-CL2  was localized in the oocyte of the ovary. Injection of  Mn-CL2  dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of  Mn-CL2  dsRNA on ovary maturation. These results suggest that  Mn-CL2  has a key role in promoting ovary maturation.

Molecular Characterization of a Novel Cathepsin L in Macrobrachium nipponense and Its Function in Ovary Maturation