English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Acinetobacter baumannii  is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) is a marker of activated type VI secretion system (T6SS), a key secretory system that promotes Gram-negative bacteria colonization, adhesion, and invasion of host cells. Hcp is also regulated by iron ions (Fe). In this study, an ATCC17978  hcp  deletion strain (ATCC17978Δ hcp ), an  hcp  complement strain (ATCC17978Δ hcp +  ), and an  A. baumannii –green fluorescent protein (GFP) strain were constructed and used to investigate the role of  hcp  in bacterial adhesion to cells (human pulmonary alveolar epithelial cells (HPAEpiC)) and biofilm formation. Our results indicate that the inhibitory concentrations of the three  A. baumannii  strains (ATCC17978 wild type, ATCC17978Δ hcp , and ATCC17978Δ hcp  + ) were drug-sensitive strains.  A. baumannii hcp  gene and iron ions might be involved in promoting the formation of a biofilm and host–bacteria interaction. Iron ions affected the ability of  A. baumannii  to adhere to cells, as there was no significant difference in the bacterial numbers when assessing the adhesion of the three strains to HPAEpiC in the presence of iron ion concentrations of 0 μM ( F  = 3.1800,  p  = 0.1144), 25 μM ( F  = 2.067,  p  = 0.2075), 100 μM ( F  = 30.52,  p  = 0.0007), and 400 μM ( F  = 17.57,  p  = 0.0031). The three strains showed significant differences in their ability to adhere to HPAEpiC. The numbers of bacteria adhesion to HPAEpiC were ATCC17978Δ hcp &amp;gt;ATCC17978Δ hcp  + &amp;gt;ATCC17978 in descending order.  Hcp  gene was positively regulated by iron ions in the bacteria–cells’ co-culture. It is speculated that the effect of iron ions on the interaction between  A. baumannii  and HPAEpiC might be related to the transport function of  hcp  and bacterial immune escape mechanisms.

frontiers in cellular and infection microbiology

Effect of Hcp Iron Ion Regulation on the Interaction Between Acinetobacter baumannii With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation