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Klebsiella pneumoniae  carbapenemase genes ( bla  KPC ) play an important role in carbapenem-resistant  Enterobacteriaceae  in China. A rapid detection method for  bla  KPC  genes and investigations into the molecular characteristics of  bla  KPC  positive  Klebsiella pneumoniae  were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for  bla  KPC  was established. This protocol could be completed at 39°C in 15–20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The  bla  KPC  RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34  bla  KPC  positive isolates were identified from each sample, of which 23 isolates were  K. pneumoniae . ST11 with  bla  KPC-2  was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of  bla  KPC  positive carbapenem-resistant  K.pneumoniae  poses significant challenges for public health control.

frontiers in cellular and infection microbiology

A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae