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In  Vibrio parahaemolyticus , type III secretion system 1 (T3SS1) is a major virulence factor that delivers effectors into the host eukaryotic cytoplasm; however, studies on its infection mechanism are currently limited. To determine the function of the  vscF  gene, we constructed the  vscF  deletion mutant Δ vscF  and complementation strain CΔ vscF . Compared with those of wild-type POR-1 and CΔ vscF , the cytotoxic, adherent, and apoptotic abilities of Δ vscF  in HeLa cells were significantly reduced ( P  < 0.01). Furthermore, in infected HeLa cells, the mutant strain reduced the translocation rates of VP1683 and VP1686 effectors compared to the wild-type and complementation strains. A BLAST search showed that  vscF  is homologous to the MixH needle protein of  Shigella flexneri , indicating that the  vscF  gene encodes the needle protein of T3SS1 in  V. parahaemolyticus . Additional translocation assays showed that VPA0226 translocated into the HeLa eukaryotic cytoplasm  via  T3SS1, secretion assays showed that VPA0226 can be secreted to supernatant by T3SS1, indicating that VPA0226 belongs to the unpublished class of T3SS1 effectors. In conclusion, our data indicate an essential role of vscF in  V. parahaemolyticus  T3SS1 and revealed that VPA0226 can be secreted into the host cell cytoplasm  via  T3SS1. This study provides insights into a previously unexplored aspect of T3SS1, which is expected to contribute to the understanding of its infection mechanism.

VscF in T3SS1 Helps to Translocate VPA0226 in Vibrio parahaemolyticus