English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Over the last decade, an increasing number of reports presented  Galleria mellonella  larvae as an important model to study host-pathogen interactions. Coherently, increasing information became available about molecular mechanisms used by this host to cope with microbial infections but few of them dealt with oxidative stress. In this work, we addressed the role of reactive oxygen species (ROS) produced by the immune system of  G. mellonella  to resist against  Salmonella enterica , an intracellular pathogen responsible for a wide range of infections. We confirmed that  Salmonella  was pathogen for  G. mellonella  and showed that it had to reach a minimal bacterial load within the hemolymph to kill the larvae. ROS production by  G. mellonella  was revealed by the virulence defects of  Salmonella  mutants lacking catalases/peroxiredoxins or cytoplasmic superoxide dismutases, both strains being highly sensitive to these oxidants. Finally, we used bacterial transcriptional fusions to demonstrate that hydrogen peroxide (H 2 O 2 ) was produced in the hemolymph of  Galleria  during infection and sensed by  S. enterica . In line with this observation, the H 2 O 2 -dependent regulator OxyR was found to be required for bacterial virulence in the larvae. These results led us to conclude that ROS production is an important mechanism used by  G. mellonella  to counteract bacterial infections and validate this host as a relevant model to study host-pathogen interactions.

Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection